A negative correlation was observed between two components of wheat yield: thousand kernel weight (TKW) and grain number per spike (GN). Nonetheless, TKW and GN were significantly higher in modern cultivars compared to landraces. Two reliable quantitative trait loci (QTLs) related to GN, QGN.nwafu-4A and QGN.nwafu-7A, were identified through genome-wide associate analysis. QGN.nwafu-4A showed pleiotropy on GN, kernel number per spikelet and spike length. B.
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