Wheat Mutants - TaGSK3 Mutants
Gene & Editing Technology Overview
The GSK3/SHAGGY kinase family is a class of serine/threonine kinases that are highly conserved in animals, plants and microorganisms and is involved in a variety of signaling pathways. GSK3 is a key negative regulatory kinase of the BR signaling pathway and is widely involved in plant growth and development. At the same time, it plays an important regulatory role in various stress responses.
These mutants are in the Chinese Spring background. They are critical materials for studying the genetic redundancy and dose effect of TaGSK3 family genes in stress tolerance and yield-related traits.
Target TaGSK3 Homoeologs
| Gene Name | Chromosome Location | Key Function |
|---|---|---|
| gsk3-1a | 1A | Salinity stress response |
| gsk3-1b | 1B | Drought stress response |
| gsk3-1d | 1D | Drought stress response |
| gsk3-3a | 3A | Cold stress response |
| gsk3-3b | 3B | Plant height regulation |
| gsk3-3d | 3D | Cold stress response |
TaGSK3 Mutant Lines by Mutation Category
All mutants are at the T3 generation, with homozygous mutations confirmed; seed count varies by line (contact for specific availability).
1. Single Mutants (1 gene inactivated)
- gsk3-1a
- gsk3-1b
- gsk3-1d
- gsk3-3a
- gsk3-3b
- gsk3-3d
2. Double Mutants (2 genes inactivated)
- gsk3-1a1b
- gsk3-1a1d
- gsk3-3a3d
- gsk3-3b3d
3. Triple Mutants (3 genes inactivated)
- gsk3-3a1b1d
- gsk3-1a1b3d
- gsk3-1a1b3a
4. Quadruple Mutants (4 genes inactivated)
- gsk3-1a1b1d3a
- gsk3-1a1b1d3d
- gsk3-1a1b3a3b
- gsk3-1a1b3a3d
- gsk3-1b3a3b3d
5. Pentuple Mutants (5 genes inactivated)
- gsk3-1b1d3a3b3d
- gsk3-1a1d3a3b3d
- gsk3-1a1b3a3b3d
- gsk3-1a1b1d3a3b
- gsk3-1a1b1d3b3d
- gsk3-1a1b1d3a3b
6. Hexuple Mutant (All 6 genes inactivated)
- gsk3(6)
Phenotype Image
Phenotypic comparison of TaGSK3 mutants (single to hexuple) and wild type (Chinese Spring) :
These are the pictures of the TaGSK3 mutants.