TaWOX5 enhances the callus induction and regeneration abilities of host tissues, allowing immature embryos under adverse physiological conditions to also produce transgenic plants, thereby overcoming the effects brought by maternal stress. Overexpression of TaWOX5 has no significant negative impact on the growth and development of transgenic plants and can serve as a marker gene, facilitating the distinction between transgenic and non-transgenic plants. The following are overexpression (OE) lines (with Fielder as the wild type), providing tools for wheat genetic transformation research.
The GRF4-GIF1 protein formed by the fusion of GRF4 (growth-regulating factor 4) and GIF1 (GRF-interacting factor 1) can significantly promote the cell division and regeneration ability of plants. In the genetic transformation research of wheat, this fusion protein can enhance the regeneration efficiency of callus, shorten the regeneration cycle, and enable the efficient genetic transformation of varieties that were originally difficult to transform. The following are overexpression (OE) lines (with Fielder as the wild type), providing tools for wheat genetic transformation research.
Wild Type Background: Fielder
Two overexpression lines, generated via Agrobacterium-mediated transformation with CaMV 35S promoter driving target gene expression, stabilized to T3 generation.
| Name (命名) | Seed Count (种子数) |
|---|---|
| Fielder-TaWOX5 overexpression strain | 500 |
| Fielder-TaGFR4-GIF1 overexpression strain | 200 |
Fielder-TaWOX5 overexpression mutants.
Fielder-TaGFR4-GIF1 overexpression mutants.
All mutants are provided for academic research only. Commercial use requires separate agreement.
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